NTML Nebraska Transposon Mutant Library Strain Primer Designer

10/2020 by Christopher F. Schuster.
No guarantee whatsoever. This includes accuracy, correctness, completeness and everything else. Use at you own risk and please do not come back to me if something didn't work out the way it should (have). Feeback is very welcome though and I will do my best to correct any mistakes I am made aware of.

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Gene name: SAUSA300_1290 NE strain: NE1118 gtcattcctaaagaatttcaagcgttatacgatgctcaacaaagtggattagatattcaagatcaatttaaaccaatcggcacactgttatcagctttatcagttgatattaacccaattcctattaaagctctaacaagttatttaggatttggaaattatgaattacgtctaccattggttagcctagaagatacagatactaaagtgcttcgtgaaacatatgacacatttaaagcgggtgaaaatgagtgaaaatattactaattggctatggcgcaatgaatcaacgcgttgctagattagcagaagaaaaaggacatgaaatcgttggggtcattgaaaatacaccgaaagcaacaacgccatatcaacaatatcaacatattgcagatgttaaaggtgccgatgttgcaatagatttttcaaatccaaatctgcttttccctttattagatgaagattttcatttgccattagttgtggcaacaactggcgagaaagaaaaactacttaataagttagatgaattgagtcaaaatatgcctgtgtttttcagcgcgaacatgagttatggcgttcatgcattgactaaaattttagcagctgctgttcccctacttgatgatttcgacatcgaattgactgaggcacatcataataaaaaagtagatgcaccaagtggtacgttagaaaaattgtatgatgtgatcgtatctttgaaagaaaatgtaacacctgtgtatgatagacatgaattaaatgaaaaacgccagccacaagatattggtatacattctattcgtggaggtacgattgtcggtgaacatgaagttctatttgctggcactgatgaaacgattcaaatcacgcatcgtgcacaatcaaaagatatttttgcgaatggtgcaatacaagcagcagaacgcttagttaataaaccaaacggcttttatacgtttgacaacttataaacatattaaaggagatcgattattttstartATGGTACAACATTTAACAGCTGAAGAAATTATTCAATATATAAGTGATGCTAAAAAGTCTACACCAATAA>>>TN>>>AAGTATATTTAAATGGTAATTTTGAAGGCATCACATATCCAGAAAGTTTTAAAGTATTTGGTTCAGAACAATCTAAAGTAATCTTTTGTGAAGCGGATGATTGGAAACCTTTTTACGAAGCATATGGTAGTCAATTCGAAGATATAGAAATTGAAATGGATCGTCGCAATTCTGCTATTCCATTAAAAGACTTAACAAATACGAATGCACGAATTGAACCAGGTGCGTTTATTAGAGAACAAGCCATTATTGAAGATGGTGCTGTCGTTATGATGGGCGCAACAATTAATATTGGCGCAGTCGTTGGCGAAGGTACAATGATTGATATGAATGCTACTCTCGGTGGTCGTGCTACAACTGGTAAAAATGTACATGTAGGGGCTGGCGCAGTATTAGCAGGTGTGATTGAACCCCCTAGTGCTTCACCGGTTATAATCGAGGATGATGTATTAATCGGTGCAAATGCAGTTATTTTAGAAGGTGTACGTGTTGGTAAAGGTGCTATTGTTGCAGCTGGCGCGATTGTGACACAAGATGTACCAGCTGGTGCAGTTGTTGCTGGTACACCTGCAAAAGTGATTAAGCAAGCTTCTGAAGTACAAGATACTAAAAAAGAGATTGTAGCAGCATTAAGAAAACTGAATGACTAGstoptgacgtcaacgtataataatttcaaggttgagatacgattatgtctcaaccttattgtttagcattgaataaacttatgatcacaaatttcgaaatacgttacactatttatatagtaatttaacacaaaggattgataattatgaatgaattagaatttgttacgaaacatcgccgtcatttacatcaacatcctgaattaagcttacatgaatttgaaacaactgcttatattaaagcgtttttagatagtttaaatattaaatacgattgcccattggaaactggcgtcattgcatacttagaaggtaatggctcacatacgatagcgtatagagctgatattgatgcgttacctattttagaggaaaatgatgtgccttatcgcagtcaatctgatcatgtgatgcatgcttgtggacatgatggtcatacaactgcattaatgctttttgtacaacgttgcaaagacatgcaagatgcaggtcaattaccgcaaaatgtcgttttcattttccaacctgcagaagaaactggtggcggtgcaaatcgattaataaaagccggtgcctttgataagtatccaattgaagcggtatttggtattcatgttaacccatttgctgatgaaggcattgcagtgataagagatgaagaaattacggccagcgcaacagagtatcgctttttcttaacaggcctgtcaagtcatgttgctgataaagaacaaggtcattcttgtggtgaagcattacaacatgtattaactcaaatatcacaaattcaacaatttcaccttaacggtttgaaacgaaatattgttcatattggtcattttaaagctggtgaagcgattaacactgtaccaagtaatggctatttagaaggtactattcgtacatatgatattgatgatttaacaatcgttaaaaatcaaatgcacaagatagcagaaagtgtcaagcttctgtttaatgtagattgtgaagttaaatttgca